INHIBITING THE KERATINOCYTE CELL CYCLE KINASES

• 48h inhibiting mitotic kinases in human skin keratinocytes irreversibly induces terminal differentiation and suppresses clonogenic potential. However,
• 48h inhibiting cell cycle entry kinase cdk2 delays differentiation and confers a higher clonogenic potential.

Further suggesting that epidermal keratinocytes need cell growth to fully differentiate.




 
Colonies of human primary keratinocytes after the treatments indicated.

Double immunofluorescence for Cyclin E (red) and the differentiation

marker involucrin (green), DNA was stained in blue by DAPI  (Upper Panels).

Note that inhibition of mitotic kinases (cdk1, Pololk) renders small, differentiated colonies,

whose cells irreversibly lose their capacity to proliferate.

Bar:100 µm. Freije et al, Oncogene, 2012, Supplementary Data.