Tuesday 3 April 2012

3D KERATINOCYTE CULTURES


3D reconstruction of confocal microscopy analyses of an in vitro colony of human primary stratifying keratinocytes. Immunofluoro-staining for the cell cycle regulator Cyclin E (green) and the differentiation marker involucrin (red); nuclear DNA counter-stained by Dapi (blue). Note that Cyclin E accumulates as cells stratify (see also Zanet et al, 2010 and Freije et al, 2012). Labelling and video by María Hidalgo.

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